2026年48（4）中英文摘要
【特約專稿】
表面增強拉曼光譜技術在中藥分析中的應用
陳萱1a,2，歐書欣1a，謝亮3，劉巖1a，章弘揚1b，江?t2，付建軍*2，張敏*1a
（1. 華東理工大學 a.藥學院，b.化學與分子工程學院，上海 200237；2. 君恒醫藥（上海）有限公司，上海 200241；3. 江西百神藥業股份有限公司 農業農村部贛產中藥質量安全監測重點實驗室，江西 宜春 336000）
摘要：中藥化學成分復雜且基質干擾嚴重，傳統分析方法在快速篩查與現場檢測中存在靈敏度不足、前處理繁瑣、貴重儀器依賴度高等局限。表面增強拉曼光譜（Surface-Enhanced Raman Spectroscopy，SERS）技術基于貴金屬納米結構的局域表面等離子體共振效應，可實現對痕量分子的信號增強與“指紋”識別，兼具高靈敏度、高特異性、快速無損、水相友好及易于便攜化集成等優勢，為中藥復雜體系的質量分析提供了新的技術途徑。系統綜述了SERS在中藥分析中的增強機制、方法特點及數據處理策略，重點闡述了其在中藥鑒別、檢查、含量測定及整體特征等方面的應用進展。同時，探討了SERS與色譜、質譜等方法聯用的互補策略及其在中藥生產全程質量監控中的潛力。隨著SERS基底制備技術的不斷成熟、標準化方法的建立以及光譜數據庫的日益完善，表面增強拉曼光譜技術有望在中藥質量控制中發揮更重要的作用。進一步推動該技術與過程分析技術和人工智能算法的融合，將為實現從藥材源頭到成品制劑的全鏈條、實時、智能質量監控提供有力工具，從而助力中藥質量的提升與產業現代化發展。
關鍵詞：表面增強拉曼光譜；中藥分析；快速檢測；質量控制；質量監測
中圖分類號：O657.3 文獻標識碼：A 文章編號：0258-3283（2026）--
DOI：10.13822/j.cnki.hxsj.2026.0023
Application of Surface-Enhanced Raman Spectroscopy in the Analysis of Traditional Chinese Medicines CHEN Xuan1a,2, OU Shu-xin1a, XIE Liang3, LIU Yan1a, ZHANG Hong-yang1b, JIANG Xuan2, FU Jian-jun*2, ZHANG Min *1a (1a. School of Pharmacy, 1b. School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai 200237, China; 2. Veritas Medicine (Shanghai) Co., Ltd., Shanghai 200241, China; 3. Key Laboratory for Quality & Safety Monitoring of Jiangxi TCM, MARA, Jiangxi Baishen Pharmaceutical Co., Ltd., Yichun 336000, China)
Abstract: The chemical composition of traditional Chinese medicines (TCMs) is complex and is often susceptible to matrix interference. Traditional analytical methods are limited by their insufficient sensitivity, cumbersome sample preparation procedures, and high reliance on expensive equipment, which render them less suitable for the rapid screening and on-site detection of TCMs. Surface-enhanced Raman spectroscopy (SERS), based on the localized surface plasmon resonance effect of noble metal nanostructures, can significantly enhance the signals of trace molecules and provide “fingerprint” identification. It combines the advantages of high sensitivity, strong specificity, rapid and non-destructive analysis, good compatibility with aqueous systems, and easy integration into portable platforms, thereby providing a new technical approach for the quality analysis of complex TCM systems. This review systematically summarized the enhancement mechanisms, methodological features, and data processing strategies of SERS in TCM analyses, focusing on the progress in the identification, inspection, content determination, and holistic characterization of TCMs. Complementary strategies that combine SERS with chromatography, mass spectrometry, and chemometric methods were also discussed, along with their potential for quality monitoring throughout the TCM production process. With the continuous advancement of the SERS substrate preparation technology, establishment of standardized methods, and improvement of spectral databases, SERS was expected to play an increasingly important role in controlling the quality of TCMs. Further integration of this technology with process analytical technologies and artificial intelligence algorithms will provide a powerful tool for realizing whole-chain, real-time, and intelligent quality monitoring of samples ranging from herbal materials to finished preparations, thereby contributing to enhanced TCM quality and the modernization of the TCM industry.
Key words：surface-enhanced Raman spectroscopy; traditional Chinese medicine analysis; rapid detection; quality control; quality monitoring
【電化學與新能源】 碳納米管增強納米硫酸鐵鈉正極材料性能 斯慶蘇都，劉德新，鄧建學，朱正偉，高艷，問研良*，洪明子* （鄂爾多斯實驗室，內蒙古自治區鄂爾多斯 017010） 摘要：鈉離子電池正極材料聚陰離子型硫酸鐵鈉(Na2Fe(SO4)2)具有理論容量高和結構穩定的優點，但較低的本征電子電導率限制了其電化學性能與規模化應用。為改善其導電性，提出通過引入高導電性的碳納米管(CNTs)，構建三維連續導電網絡，以提升材料的電化學性能。采用溶膠-凝膠法結合高溫煅燒的兩步合成策略制備純相Na2Fe(SO4)2。通過超聲輔助液相混合及后續處理工藝，將CNTs均勻附著于Na2Fe(SO4)2顆粒表面，形成Na2Fe(SO4)2/CNTs復合材料。XRD分析表明復合材料仍保持純相Na2Fe(SO4)2結構，CNTs未改變其物相純度。Na2Fe(SO4)2/CNTs復合材料通過CNTs的引入，顯著提升了電子傳輸能力和結構穩定性，通過循環后的SEM測試表明CNTs網絡緩解充放電過程中的體積應力，從而維持了顆粒的完整性，并且大幅改善電化學性能。電導率測試顯示復合材料電導率提升至10-3 S/cm量級，較純相材料提高至少3個數量級。電化學測試表明，純相材料初始放電比容量僅為67.06 mAh/g，而復合材料首圈容量提升至78.73 mAh/g，并表現出優異倍率性能（5 C下容量67.31 mAh/g）和長循環穩定性（在2~4.5 V電壓窗口循環100圈后容量保持率達78.7%）。 關鍵詞：鈉離子電池；正極材料；硫酸鐵鈉；碳納米管；電化學性能 中圖分類號：O64，TB331 文獻標識碼：A 文章編號：0258-3283（2026）-- DOI：10.13822/j.cnki.hxsj.2026.0035 Carbon Nanotube-Enhanced Performance of Sodium Iron Sulfate Nanocathode Materials SI Qing-su-du, LIU De-xin, DENG Jian-xue, ZHU Zheng-wei, GAO Yan, WEN Yan-liang*, HONG Ming-zi* (Ordos lab, Ordos 017010, China) Abstract：Polyanionic sodium iron sulfate (Na2Fe(SO4)2) cathode material for sodium-ion batteries offers advantages of high theoretical capacity and structural stability. However, its low intrinsic electronic conductivity limits both electrochemical performance and large-scale application. To enhance conductivity, we propose constructing a three-dimensional continuous conductive network by incorporating highly conductive carbon nanotubes (CNTs), thereby improving the material’s electrochemical performance. A two-step synthesis strategy combining sol-gel preparation with high-temperature calcination was employed to synthesize pure-phase Na2Fe(SO4)2. Through ultrasonic-assisted liquid-phase mixing and subsequent processing, CNTs were uniformly attached to the surface of Na2Fe(SO4)2 particles, forming Na2Fe(SO4)2/CNTs composites. XRD analysis indicates that the composite material retains the pure-phase Na2Fe(SO4)2 structure, with CNTs not altering its phase purity. The incorporation of CNTs into the Na2Fe(SO4)2/CNTs composite significantly enhances its electronic conductivity and structural stability. SEM analysis following cycling demonstrates that the CNTs network alleviates volumetric stress during charging and discharging, thereby preserving particle integrity and substantially improving electrochemical performance.Conductivity testing shows the composite material's conductivity enhanced to the 10-3 S/cm range, representing at least a three-order-of-magnitude improvement over the pure-phase material. Electrochemical testing revealed that the initial discharge specific capacity of the pure-phase material was only 67.06 mAh/g, whereas the composite material achieve a first-cycle capacity of 78.73 mAh/g, demonstrating excellent rate capability (67.31 mAh/g at 5 C) and long-term cycling stability (78.7% capacity retention after 100 cycles within the 2~4.5 V voltage window). Key words：sodium-ion battery; cathode material; sodium iron sulfate; carbon nanotubes; electrochemical performance 【化學品與環境】 氣相色譜法同時測定土壤中脂肪烴與芳香烴含量 周健，梁靜*，羅雨薇，劉永 (國檢測試控股集團京誠檢測有限公司，廣東 廣州 510000) 摘要：為建立土壤中脂肪烴與芳香烴的同步氣相色譜分析方法，系統考察了土壤樣品基質中的脂肪烴和芳香烴分離純化過程中，壓力、填料種類、洗脫溶劑類型等關鍵影響因素，并探究了樣品基質對優化后分析方法檢測結果的干擾效應，明確了該方法在土壤樣品實際檢測中的可行性。實驗稱取10 g凍干土壤樣品，采用加壓流體萃取法進行萃取，經凈化除雜、濃縮處理后獲得凈化液；選取硅膠小柱作為分離柱，先以正己烷為洗脫溶劑收集脂肪烴組分，再加入二氯甲烷繼續洗脫得到芳香烴組分，將兩組分分別濃縮、定容、過濾后，采用GC-FID進行定性定量分析。結果表明，石油烴經硅酸鎂柱凈化后，在常壓條件下選用SPE硅膠小柱作為分離柱，以正己烷、二氯甲烷依次梯度洗脫，可實現脂肪烴與芳香烴的高效分離純化，且方法受樣品基質干擾較小。基質加標試驗結果回收率范圍為80.9%~95.3%，相對標準偏差范圍為1.11%~11.2%，該方法優化了土壤中兩類烴類化合物的前處理流程與分離參數，操作簡便、分離效果良好，為土壤環境中脂肪烴和芳香烴的精準檢測提供了可靠的技術支撐，適用于土壤烴類污染監測與風險評估工作。 關鍵詞：氣相色譜法；脂肪烴；芳香烴；石油烴；分離純化 中圖分類號：O657.7；X833 文獻標識碼：A 文章編號：0258-3283（2026）-- DOI：10.13822/j.cnki.hxsj.2025.0299 Simultaneous Determination of Aliphatic and Aromatic Hydrocarbons in Soil by Gas Chromatography ZHOU Jian, LIANG Jing*, LUO Yu-wei，LIU Yong (Guojian Testing Holding Group Jingcheng Testing Co., Ltd.,Guangzhou 510000, China ) Abstract: To establish a synchronous gas chromatographic analysis method for aliphatic and aromatic hydrocarbons in soil, the effects of key factors, such as pressure, packing material, and elution solvent, on the separation and purification of these hydrocarbons in the soil matrix were systematically investigated. In addition, the interference effect of the sample matrix on the detection results of the optimized analytical method was evaluated, thereby confirming the feasibility of the proposed method for the analysis of actual soil samples. In the experiment, 10 g of a freeze-dried soil sample was subjected to pressurized fluid extraction. A purified solution was obtained after purification, impurity removal, and concentration steps. A silica gel column was used for separation. First, n-hexane was used as the elution solvent to collect the aliphatic hydrocarbon fraction, and dichloromethane was then added to continue elution and obtain the aromatic hydrocarbon fraction. The two fractions were separately concentrated, diluted to a fixed volume, filtered, and subjected to qualitative and quantitative analyses by GC-FID. The results indicate that after purification of petroleum hydrocarbons with magnesium silicate columns, efficient separation and purification of aliphatic and aromatic hydrocarbons can be achieved under atmospheric pressure using SPE silica gel cartridges as the separation column and sequential elution with n-hexane and dichloromethane in a gradient mode; this method is less affected by sample matrix interference. The recovery percentage derived from the results of the matrix spike test ranged from 80.9% to 95.3%, and the relative standard deviation ranged from 1.11% to 11.2%. The proposed GC-FID analytical method optimizes the pretreatment process and separation parameters for the two types of hydrocarbon compounds in soil. Overall, the method is simple to operate, has a good separation effect, and provides reliable technical support for the accurate detection of aliphatic and aromatic hydrocarbons in soil. This analytical method applies to soil hydrocarbon pollution monitoring and risk assessment. Key words: gas chromatography; aliphatic hydrocarbons; aromatic hydrocarbons; petroleum hydrocarbons; separation and purification 氣質多反應監測在西寧市果蔬農殘檢測中的應用 李玉環，馬春光*，王怡，李增明*，甘瑛琳，楊增亮，張敏 （西寧市疾病預防控制中心，青海西寧 810000） 摘要：建立基質固相分散技術及氣相色譜-質譜聯用儀多反應監測（MRM）模式在西寧市當地蔬菜、水果中前處理技術和檢測技術的方法并評估其應用效果，明確該技術組合的實用性與可靠性，并建立西寧市當地蔬菜、水果中農藥殘留的高效檢測方法，提升農產品質量安全監管水平。研究以西寧市五區兩縣主流農貿市場、商超的多品類果蔬為研究對象，采用基質固相分散技術處理樣品，結合MRM模式建立了13種農藥殘留的檢測方法。方法在0～5mg/kg濃度范圍內線性關系良好（相關系數R2為0.9983～0.9996），檢出限、定量限分別為0.003～0.05、0.009～0.15 μg/kg，加標濃度50、100 μg/L時，回收率分別達82.88%～96.74%、81.27%～97.72%，精密度分別為1.24%～4.72%、3.05%～6.92%，各項性能指標均符合農藥殘留檢測技術規范，具備高靈敏度、高準確性和良好穩定性的優勢。方法操作簡便、檢測高效，可有效適配西寧市多品類果蔬的13種農藥殘留檢測，成功填補了青藏高原地區相關檢測技術空白，為當地農產品質量安全監管提供了可靠的技術支撐。但該方法僅涉及13種農殘，仍存在檢測農藥種類有限、且缺少高濃度點的回收、精密度等的數據分析，有待后續研究進一步完善。 關鍵詞：農藥殘留；氣質聯用；多反應監測；蔬菜；水果 中圖分類號：O65 文獻標識碼：A 文章編號：0258-3283（2026）-- DOI：10.13822/j.cnki.hxsj.2026.0002 Multiple Reaction Monitoring for Detecting Pesticide Residues in Vegetables and Fruits from Xining City LI Yu-huan, MA Chun-guang*, WANG Yi, LI Zeng-ming*, GAN Ying-lin, YANG Zeng-liang, ZHANG Min (Xining City Center for Disease Control and Prevention, Xining 810000, China) Abstract: A method for pretreating and detecting pesticide residues in vegetables and fruits acquired from Xining City was established by leveraging matrix solid-phase dispersion (MSPD) combined with gas chromatography-tandem mass spectrometry (GC-MS/MS) in the multiple reaction monitoring (MRM) mode. The applicability, practicality, and reliability of this combined technique were evaluated, with the ultimate objective of enhancing the quality and safety supervision of agricultural products. Common vegetable and fruit samples (including leafy, root, stem, and berry types) were collected from major farmers’ markets and supermarkets across the five districts and two counties of Xining. After MSPD treatment, the extracts were analyzed using GC-MS/MS in the MRM mode to determine the levels of 13 pesticide residues. The detection range was 0～5 mg/kg. The proposed method demonstrated good linearity, with correlation coefficients between 0.9983 and 0.9996. The limits of detection ranged from 0.003 to 0.05 μg/kg, and the limits of quantification ranged from 0.009 to 0.15 μg/kg. At spiked concentrations of 50 and 100 μg/L, the recovery rates were 82.88%～96.74% and 81.27%～97.72%. The corresponding precision values, expressed as relative standard deviations, were 1.24%～4.72% and 3.05%～6.92%. The MSPD technique simplifies sample preparation, reduces organic solvent consumption, and lowers environmental impact and cost. The GC-MS/MS method in the MRM mode offers high specificity, eliminates matrix interference, and improves sensitivity and accuracy. Overall, this combined approach demonstrates significant advantages for large-scale screening of pesticide residues in fruits and vegetables in Xining. It offers enhanced detection efficiency and reliable results, providing strong technical support for ensuring the quality and safety supervision of local agricultural products. Keywords: pesticide residues; gas chromatography-tandem mass spectrometry; multiple reaction monitoring; vegetables; fruits 【分析與測試】 ICP-AES法測定鑄鋁合金全元素的溶樣優化與準確度驗證 張亮亮*1，袁琪1，李婭娟2，謝培均1 （1. 中航金屬材料理化檢測科技有限公司，陜西 西安 710000；2. 西部超導材料科技股份有限公司，陜西 西安 710000；3. 中航金屬材料理化檢測科技有限公司，貴州 安順 561000） 摘要：針對鑄鋁合金（低硅Si<0.50%、高硅Si:4.0%~10.0%）全元素精準分析的需求，聚焦高硅體系溶樣難題與多元素檢測干擾控制，通過系統優化前處理條件、儀器參數及干擾消除方法，建立了高效可靠的ICP-AES分析技術。實驗明確兩類合金最優方案：低硅鑄鋁合金以0.10 g為稱樣量，采用15 mL（6mol/L）鹽酸 在70℃溶解；高硅鑄鋁合金以0.05 g為稱樣量，通過5 mL體積分數50%硝酸 與1 mL氫氟酸混合體系實現完全溶解，有效規避硅元素損失與膠體膜生成問題。通過基體匹配校準抵消鋁基體效應，優化分析線選擇與酸度控制消除光譜及化學干擾，構建的校準曲線對Si、Fe、Cu、Mn、Ti、Zr、Ni、Sb、Co、Mg、Pb、Sn、Zn、Be等14種目標元素的線性相關系數R2≥0.995，檢出限低至0.001~0.012 mg/L。準確度驗證顯示，低硅與高硅鑄鋁合金標準樣品的測定值與標準值高度吻合，相對標準偏差（RSD）分別介于0.197%~3.26%和0.19%~4.87%之間。方法簡化了前處理流程，突破了高硅鑄鋁合金全元素同步檢測的技術瓶頸，兼顧準確性與實用性，為鑄鋁合金質量控制及成分溯源提供了全面的技術支撐。 關鍵詞：鑄鋁合金；ICP-AES；全元素檢測；溶樣優化；基體匹配；準確度驗證 中圖分類號：O657.31 文獻標識碼：A 文章編號：0258-3283（2026）-- DOI：10.13822/j.cnki.hxsj.2025.0111 Optimization of Sample Dissolution and Accuracy Verification for Multi-Element Determination in Cast Aluminum Alloy by ICP-AES ZHANG Liang-liang*1, YUAN Qi1, LI Ya-juan2, XIE Pei-jun1 (1. AVIC Metal Test Technology Co., Ltd., Xi'an 710000, China; 2. Western Superconducting Technologies Co., Ltd., Xi'an 710000, China; 3. AVIC Metal Test Technology Co., Ltd., Anshun 561000, China) Abstract: To address the need for accurate full-element analysis of cast aluminum alloys, including low-silicon (Si<0.50%) and high-silicon (4.0%~10.0% Si) systems, this study focused on overcoming sample dissolution challenges in high-silicon alloys and minimizing multi-element interference during detection. An efficient and reliable ICP-AES method was developed through systematic optimization of sample pretreatment procedures, instrumental parameters, and interference control strategies. Experimental optimization established the following protocols: for low-silicon cast aluminum alloys, 0.10 g of sample was dissolved in 15 mL of 6 mol/L hydrochloric acid at 70 ℃; for high-silicon cast aluminum alloys, 0.05 g of sample was completely dissolved using a mixed acid system consisting of 5 mL of volume fraction 50% nitric acid and 1 mL of hydrofluoric acid. This approach effectively prevented silicon loss and avoided colloidal film formation. Matrix effects from the aluminum base were compensated using matrix-matched calibration, while spectral and chemical interferences were minimized through optimized analytical line selection and acidity control. The calibration curves for 14 target elements (Si, Fe, Cu, Mn, Ti, Zr, Ni, Sb, Co, Mg, Pb, Sn, Zn, and Be) demonstrated excellent linearity (R2≥0.995), with limits of detection ranging from 0.001 to 0.012 mg/L. Accuracy validation showed strong agreement between measured and certified values for both low- and high-silicon reference materials; the relative standard deviations ranged from 0.197% to 3.26% and 0.19% to 4.87%, respectively. The proposed method simplifies sample pretreatment, overcomes key technical challenges in simultaneous full-element determination of high-silicon cast aluminum alloys, and achieves s balance between analytical accuracy and practical applicability. It provides robust technical support for quality control and compositional traceability of cast aluminum alloys. Key words: cast aluminum alloy; ICP-AES; full-element detection; sample dissolution optimization; matrix-matched calibration; accuracy validation 基于UPLC-Q-Exactive-Orbitrap-MS技術分析丹黃膏的 化學成分 趙勝*，王力欣，余青青，夏進，鄭炯，楊利，趙美，張波 （仁懷市中醫院 代謝內分泌科 貴州 仁懷 564500） 摘要：采用超高效液相色譜-四極桿-靜電場軌道阱高分辨質譜（UPLC-Q-Exactive-Orbitrap-MS）技術對丹黃膏的化學成分進行快速的定性鑒別。色譜采用Waters ACQUITY UPLC HSS T3 C18色譜柱，以0.1%甲酸-水溶液（A相）和甲醇（B相）為流動相，流速0.3 mL/min、柱溫40℃和進樣量2 μL；質譜采用電噴霧離子源（ESI），在正、負離子模式下進行全掃描/數據依賴二級掃描 （Full MS/dd-MS2）。將采集得到的化合物的保留時間、準分子離子、特征碎片離子與文獻報道的化合物或對照品進行比對，對丹黃膏的化學成分進行快速定性鑒別。共鑒定172個化合物，包括倍半萜類48個、黃酮類32個、醌類24個、苯丙素類15個、有機酸類14個、糖類9個、苯酞類7個、氨基酸類5個及其他成分18個。UPLC-Q-Exactive-Orbitrap-MS技術可快速定性鑒別丹黃膏的化學成分，該結果為丹黃膏進一步的藥效物質基礎、作用機制及產品質量控制研究供參考依據。 關鍵詞：超高效液相色譜-四極桿-靜電場軌道阱質譜法；丹黃膏；化學成分；倍半萜類；醌類；裂解途徑 中圖分類號：O657，R282 文獻標識碼: A 文章編號：0258-3283（2026）-- DOI: 10.13822/j.cnki.hxsj.2025.0320 Identification of Chemical Constituents in Danhuang Ointment Based on UPLC-Q-Exactive-Orbitrap-MS ZHAO Sheng*, WANG Li-xin, YU Qing-qing, XIA Jin, ZHENG Jiong, YANG Li, ZHAO Mei, ZHANG Bo (Department of Endocrinology and Metabolism, Renhuai Hospital of Traditional Chinese Medicine, Renhuai 564500, China) Abstract: Rapid qualitative identification of the chemical constituents of Danhuang Ointment was performed using UPLC-Q-Exactive-Orbitrap-MS. Chromatographic separation was carried out on a Waters ACQUITY UPLC HSS T3 C18 column using 0.1% formic acid-water solution (phase A) and methanol (phase B) as the mobile phase at a flow rate of 0.3 mL/min with a column temperature of 40 °C, and an injection volume of 2 μL. Mass spectrometry was performed using an electrospray ionization (ESI) source in both positive and negative ion modes for full-mass scanning/data-dependent secondary scanning (Full MS/dd-MS2). The retention times, quasi-molecular ions, and characteristic fragment ions of the compounds obtained from the analysis were compared with those reported in the literature and with the reference standards for rapid qualitative identification of the chemical constituents in Danhuang Ointment. A total of 172 compounds were identified, including 48 sesquiterpenoids, 32 flavonoids, 24 quinones, 15 phenylpropanoids, 14 organic acids, nine saccharides, seven phthalides, 5 amino acids, and 18 other compounds. The UPLC-Q-Exactive-Orbitrap-MS technique enabled rapid qualitative identification of the chemical constituents of Danhuang Ointment. These results provide reference information for further research on the pharmacodynamic material basis, mechanisms of action, and quality control of Danhuang Ointment. Key words: UPLC-Q-Exactive-Orbitrap-MS; Danhuang Ointment; chemical constituents; sesquiterpenoids; quinones; fragmentation pathways 基于DTNB法的還原型谷胱甘肽檢測試劑優化研究 孫鑫，夏云豪，臧榮斌，王永旗，呂明，盧洪慶* (太倉滬試試劑有限公司，江蘇蘇州 215421) 摘要：谷胱甘肽（GSH）是一種含活性巰基的內源性三肽，在機體抗氧化防御與細胞代謝調控中具有關鍵作用，其含量可通過Ellman試劑與巰基的特異性反應進行測定。在適宜條件下，該反應生成黃色產物2-硝基-5-巰基苯甲酸（TNB-），其在412 nm波長處具有特征吸收峰，吸光度值與樣品中GSH的濃度呈正比關系。本研究利用GSH與DTNB的特異性反應，在優化實驗條件的基礎上，測定反應產物在412 nm波長處的吸光度值，并通過標準曲線法對樣品中的GSH含量進行定量分析。在優化的實驗條件下，GSH濃度在3.125～800 μg/mL范圍內與吸光度值呈現良好的線性關系（R2 = 0.9999），方法的檢出限（LOD）為2.400 μg/mL；相對標準偏差（RSD）≤5%，加標回收率在95%～105%之間。所建立的還原型谷胱甘肽檢測試劑靈敏度高、重復性好、準確度高，基于該方法研制的谷胱甘肽檢測試劑操作簡便快捷，為相關樣本中還原型谷胱甘肽的定量分析提供了可靠的檢測手段。 關鍵詞：谷胱甘肽；DTNB法；分光光度法；檢測限；回收率 中圖分類號：O65 文獻標識碼：A 文章編號：0258-3283（2026）-- DOI：10.13822/j.cnki.hxsj.2026.0030 Reagent Optimization for Reduced Glutathione Determination Based on the DTNB Method SUN Xin, XIA Yun-hao, ZANG Rong-bin, WANG Yong-qi, LV Ming, LU Hong-qing* (Taicang Hushi Reagent Co., Ltd., Suzhou 215421, China) Abstract： Glutathione (GSH) is an endogenous tripeptide containing an active sulfhydryl group, playing a crucial role in the body's antioxidant defense and cellular metabolism regulation. Its content can be determined through the specific reaction between Ellman’s reagent and thiol groups. Under appropriate conditions, this reaction yields a yellow product, 2-nitro-5-mercaptobenzoic acid (TNB-), which exhibits a characteristic absorption peak at 412 nm, and the absorbance value is proportional to the concentration of GSH in the sample. Based on the specific reaction between glutathione and DTNB, the absorbance of the reaction product was measured at a wavelength of 412 nm under optimized experimental conditions. The GSH content in the samples was quantitatively analyzed using the standard curve method. Under the optimized experimental conditions, the GSH concentration exhibited a good linear relationship with the absorbance value within the range of 3.125 to 800 μg/mL (R2 = 0.9999). The limit of detection (LOD) of the method was 2.400 μg/mL. The relative standard deviation (RSD) was ≤5%, and the spiked recovery rates ranged from 95% to 105%. The established DTNB method demonstrates high sensitivity, good reproducibility, and high accuracy. The glutathione detection reagent developed based on this method is simple and rapid to operate, providing a reliable means for the quantitative analysis of reduced glutathione in relevant samples. Key words: glutathione; DTNB method; spectrophotometry; limit of detection; recovery
alkaline hydrolysis; lignin; cellulose
